China,Henan province,Zhengzhou Zhengzhou Tobacco Research Institute of CNTC Zhengzhou, Henan, China (People's Republic)
Body of Abstract: Application of carbon nanosol (CNs) in agricultural production has triggered the interest of researchers. However, despite the enormous importance of CNs in plant development, little is known about the effects of carbon nanoparticle (CNs) doses on plant growth responses and the underlying physiological and molecular mechanisms. In this study, we found that a low concentration (0.3%, w/v) CNs promotes the growth of tobacco plants under hydroponic culture system, which were inhibited by a high CNP concentration (1.0%). Root architecture system analysis showed 0.3% CNs had a positive effect on the elongation of primary roots and the proliferation of lateral roots. ICP-MS assisted ionomics revealed 0.3% CNs did not significantly alter the homeostasis of essential mineral nutrients. Further, high performance liquid chromatography showed the JA and SA concentrations were obviously reduced in the shoots under 0.3% CNs, which significantly triggered the IAA accumulation in the roots. Non-invasive micro-test technique also demonstrated an obvious influx of auxin in the root elongation zone. High-throughput comparative transcriptomics profiling showed that the genes involved in the responses to external stimuli and photosynthesis were highly enriched. Moreover, the genes implicated in the biosynthesis and polar transport of auxin were significantly upregulated under 0.3% CNs compared with the control. Overall, this study suggests the effectiveness of CNs as a promising approach to enhance the growth of tobacco plants through regulating the auxin homeostasis.