Body of Abstract: Flavins are crucial metabolites that have roles in almost every aspect of plant physiology. They are organic, water-soluble compounds that easily accept and donate 1 or 2 electrons, making them ideal cofactors for numerous biological processes in all organisms including DNA repair, light sensing, protein folding, and more. Riboflavin (RF), known as vitamin B2, is the precursor to two major flavin cofactors: flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Flavin biosynthesis in plants occurs through a conserved pathway, the regulation of which is still unknown. Based on preliminary data identifying potential candidate genes involved in regulation of flavin homeostasis in plants, we believe two long noncoding RNAs are potentially involved in flavin homeostasis. Elucidation of this pathway is a key step to improving crop nutritional quality and ensuring that regions that rely on plants for sustenance avoid vitamin B2 deficiency. Altered expression of key genes in the flavin biosynthetic pathway does not change flavin levels to the degree we would expect; this indicates that other genes are at work to maintain flavin homeostasis. RNAseq analysis on transgenic plants predicted to have increased RF content revealed significantly increased expression of two long noncoding RNAs (lncRNAs). lncRNAs are poorly studied in plants, but have already been established to have critical roles in regulating basic metabolic processes. Preliminary experiments completed in our lab on these two lncRNAs indicate that suppression of one of these, named PREVENTION OF RIBOFLAVIN ACCUMULATION (PORA), leads to significantly increased flavin contents in Arabidopsis seeds compared to wild type, indicating it may play a role in flavin homeostasis. Suppression of the second lncRNA, named BIG BABY PLANT (BBP), led to significantly larger plantlets through a currently unknown mechanism. Our results indicate that these lncRNAs may have important roles in crop improvement, both in relation to nutrition and biomass.