Body of Abstract: The fruits of domesticated tomato (Solanum lycopersicum) are a major food crop with a wide range of shapes and sizes that influence consumer selection. Most of the variations in fruit shape can be explained by SUN, OVATE, SOV1, FS8.1, GLOBE, LC, and FAS genes. A loss-of-function mutation in ovate, the founding member of the OVATE Family Protein (OFP) class, results in elongated fruit shape in tomato. ovate interacts with the sov1 and sov2 loci that suppress the elongated fruit shape. sov1 encodes another member of the OFP class, SlOFP20. However, sov2 remains to be finemapped. Therefore, the objective of this study is to map the location of sov2 on chromosome 11 more precisely. To do so, the heirloom tomato variety Yellow Pear, which has ovate, sov1 and sov2 mutations, was crossed with a fruit shape near isogenic line, SA38 (LA1589ovate/sov1). Selected recombinant seedlings at the sov2 locus were selected, where a set of seedlings carrying the homozygous allele for each parental genotype were transplanted in the greenhouse. A total of 12 different progeny families were grown to maturity and 12 representative fruits were harvested from each plant. Fruits were cut longitudinally and scanned at 300 dpi on a flatbed scanner. The scans were then analyzed using Tomato Analyzer 4.0 and evaluated for fruit shape index as well as proximal and distal end angles (micro and macro). Combined, the progeny testing results suggest that sov2 is further finemapped to a 357,768 bp interval on chromosome 11. This work is supported by NSF IOS 2048425. Presentation of this work at the Plant Biology 2023 conference is supported by the University of Georgia Department of Plant Biology’s Alan Jaworski Student Travel Award.