Body of Abstract: Brome mosaic virus (BMV) is a positive-strand RNA virus that primarily infects monocots, such as barley, wheat, and rice. BMV is used as a model virus due to its small genome, robust replication, and ability to replicate in the baker's yeast. We use the BMV-yeast system to learn more about viral replication mechanisms and virus-host interactions. Here we show that the host factor Lam5 (Lipid transfer at membrane contact sites 5) is involved in BMV replication. We have found that Lam5 is relocalized by BMV to the viral replication sites in yeast cells. Furthermore, we show that BMV replication in the lam5Δ mutant is drastically less than that of wild-type yeast. The doubling-time of the lam5Δ mutant is much faster than that of wild-type yeast, indicating a difference in cell viability. Furthermore, when we express Arabidopsis Lam2 tagged with GFP in yeast cells, we see a difference in localization of this protein when BMV is expressed, giving us an interesting target for gene silencing by VIGS. This research contributes to our understanding of how BMV, a model virus, uses its host to replicate.