(300-34) Metatranscriptome analysis reveals the possible role of an endophyte from salt-adapted Pokkali rice in mediating tolerance to salinity stress in salt sensitive rice variety.
Post Doctoral Fellow (Current Position) Indiana University Bloomington, Indiana
Body of Abstract: Metatranscriptome analysis reveals the possible role of an endophyte from salt-adapted Pokkali rice in mediating tolerance to salinity stress in salt sensitive rice variety.
Megha H Sampangi Ramaiah1, 2, and R. Uma Shaanker1, 2, 3
1School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bangalore 560065, India
2Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bangalore 560065, India
3Present address: Department ofBioscience and Bioengineering, Indian Institute of Technology Jammu, Jagti, Nagrota Bypass Road, Jammu & Kashmir, 181221, India.
ABSTRACT:
In our previous study, we showed that a salt tolerant endophyte, Fusarium sp., isolated from the salt adapted Pokkali rice, was able to successfully colonize and confer salinity stress tolerance to salt sensitive paddy genotype, IR-64. Comparative transcriptome analysis of plants enriched with the endophyte (E+S+) over those not enriched (E-S+), under salinity stress, revealed 1348 upregulated and 1078 down regulated genes. But, the role of the microbiome in the above conditions were still unknown. Thus, in the current study we investigated the micro flora of the above treatments by a metatranscriptome analysis. Co-occurrence analysis indicated that bacteria were less firmly linked with each other compared to fungi and archaea. Fungi were found to be most closely interconnected with each other compared to bacteria and archaea. It was also observed that microbial Na+/K+ transporters, Serine-Threonine kinases, BTB, FBOX genes were upregulated. Further, their KEGG pathway analysis revealed that microbial β-alaine biosynthesis, Brassinosteroids biosynthesis, Terpenoid backbone biosynthesis and Ca2+ signaling were up regulated with the introduction of salt tolerant pokkali endophyte.