Body of Abstract: Ethylene plays its essential roles in plant development, growth, and defense response by controlling the transcriptional reprograming, in which EIN2-C-directed regulation of histone acetylation is the key step for chromatin to perceive ethylene signaling. But how the nuclear acetyl CoA is produced to ensure ethylene-mediated histone acetylation is unknown. Here we report that ethylene triggers pyruvate dehydrogenase complex (PDC) to translocate from the mitochondria to the nucleus to synthesize nuclear acetyl CoA for EIN2-C-directed histone acetylation regulation, and thereafter transcriptional regulation. PDC is identified as an EIN2-C nuclear partner, and ethylene triggers its nuclear accumulation. Mutations in PDC lead to an ethylene-hyposensitivity that results from the reduction of histone acetylation and transcription activation. The nuclear PDC is enzymatically active to synthesize nuclear acetyl CoA for EIN2-C-directed histone acetylation. These findings uncover a mechanism by which PDC-EIN2 converges the mitochondrial enzyme mediated nuclear acetyl CoA synthesis with epigenetic and transcriptional regulation for plant hormone response.