Agronomist Florestaria Cantareira LTDA São Paulo, Sao Paulo, Brazil
Body of Abstract: The soil-plant-atmosphere factor directly interferes with the development and productive capacity of food plants. This work aimed to biostimulate the culture of Phaseolus vulgaris with abiotic factors that cause physiological stress, which cause lower productivity, in acidic, saline and low water availability environments, for biochemical analysis and quantification of the synthesis of amino acids that synthesize resistances with chemical and biochemical inputs. The test consists of growing to the “R5” phenological stage, at which time the treatments were stressed in three different and separate conditions: acid solution, saline solution and under water reduction. The products selected for biostimulation were CROP+™ (5 mL.L-1) and Phylgreen® (5 mg.L-1) whose composition has seaweed extract, formulated with chelated micronutrients and the product ZN+Mn® (2 mg.L-1). In each treatment there were four applications of the products, with the exception of the controls (T1, T3 and T5), after which samples were collected from the leaf part of the plants for biochemical analysis. Through laboratory methods, the recovery of quaternary ammonium (QAC) related to glycine betaine (GB), the main amino acid of response under plant stress, was carried out. By means of a spectrophotometer, the collected data were submitted to Fisher's exact test at 5% of significance in the ANOVA. The treatment that obtained the best synthesis of amino acids was submitted to stress in an acidic environment (T2). Then, in a saline environment (T4). The treatments, in relation to water deficit, did not obtain a positive result. The CROP+™ input can be indicated for use by bean farmers to relieve plant stress in saline and acidic environments. The result obtained in an environment with water deficit indicates the genetic resistance of the bean plant to this type of stress, the antagonistic production of the synthesis, and also the potential for the isolation of QAC.